Beijing's relationship with Tokyo has suffered since conservative Prime Minister Sanae Takaichi took office in October.
Ties with Ottawa have also encountered turbulence, although tensions have eased more recently with Canada and China striking a trade deal in January.
China's commerce ministry began imposing preliminary duties of up to 40.5 percent on halogenated butyl rubber after reaching initial findings last year that shipments from Japan and Canada had harmed the domestic industry.
Halogenated butyl rubber is used in various items, including tyre linings, hoses and pharmaceutical products.
Investigators issued a "final determination" on Friday, concluding that the Chinese industry "has suffered material injury, and that a causal link exists (with) the dumping", the commerce ministry said.
Such shipments from Japanese firms will be subject to tariffs of 30.1 percent from Saturday, apart from Japan Butyl, which will be given a lower levy of 15 percent, it said.
All Canadian companies will receive a 13.8 percent tariff, including major producer Arlanxeo, the ministry said.